CHARACTERISTIC OF FROZEN-THAWED EPIDIDYMAL SPERMATOZOA AND REFRIGERATED STORAGE OF RAM SPERMATOZOA
نویسندگان
چکیده
منابع مشابه
Effects of storage duration at 5 ºC and type of cryoprotectant on cooled and frozen-thawed ram epididymal spermatozoa
Freezing of ejaculated and epididymal spermatozoa is currently a subject of interest with the purpose of establishing an efficient gene banking model for valuable animals or endangered species. Therefore, the present study evaluated the influence of different storage duration )0, 1.5 and 5 h) at 5 ºC and type of cryoprotectant on freezability of ram epididymal spermatozoa. With increasing the s...
متن کاملDevelopmental competence of Dromedary camel oocytes fertilized in vitro by frozen-thawed ejaculated and epididymal spermatozoa
The present study aimed to compare the in vitro fertilizing capacity of frozen-thawed ejaculated and epididymal spermatozoa in order to standardize the semen preparation protocol for camel in vitro fertilization (IVF). Semen samples were collected from 7 Dromedary camels by means of artificial vagina (AV). Ten cauda epididymes were obtained from slaughtered adult camels, isolated, incised and r...
متن کاملThe effect of seminal plasma on the quality of coated ram frozen-thawed spermatozoa
Two experiments were designed to examine the effects of crude seminal plasma (CSP) exposure ofejaculated and epididymal spermatozoa before freezing. Experiment 1, two consecutive ejaculates werecollected (n=4) within 5 min, the second one was carried out in a tube containing Fiser extender (coatedspermatozoa); by centrifugation, seminal plasma was removed from coated ejaculates. The pellets wer...
متن کاملLeptin Receptor in Ram Epididymal Spermatozoa
This experiment was designed to investigate leptin receptors (Ob-R and Ob-Rb) mRNA in ram epididymal spermatozoa by RT-PCR. Ten testes were obtained from abattoir and epididymal spermatozoa recovery was performed. To purify spermatozoa, motile sperm were isolated by the swim-up procedure. Total RNA was isolated from epididymal spermatozoa and placental cotyledon, as a positive control, and then...
متن کاملDevelopmental competence of Dromedary camel oocytes fertilized in vitro by frozen-thawed ejaculated and epididymal spermatozoa
The present study aimed to compare the in vitro fertilizing capacity of frozen-thawed ejaculated and epididymal spermatozoa in order to standardize the semen preparation protocol for camel in vitro fertilization (IVF). Semen samples were collected from 7 Dromedary camels by means of artificial vagina (AV). Ten cauda epididymes were obtained from slaughtered adult camels, isolated, incised and r...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of the Indonesian Tropical Animal Agriculture
سال: 2010
ISSN: 2460-6278,2087-8273
DOI: 10.14710/jitaa.35.1.63-67